Cytodiagnosis of Extraskeletal Ewing’s Sarcoma and its Confirmation by Fluorescence in situ Hybridization
Published: August 1, 2016 | DOI: https://doi.org/10.7860/JCDR/2016/20155.8317
Biswajit Dey, Ashish Ranjan Singh, Adarsh Barwad, Prasad Dange, Neelaiah Siddaraju
1. Senior Resident, Department of Pathology, Jawaharlal Institute of Postgraduate Medical Education and Research, Pondicherry, India.
2. Junior Resident, Department of Pathology, Jawaharlal Institute of Postgraduate Medical Education and Research, Pondicherry, India.
3. Assistant Professor, Department of Pathology, Jawaharlal Institute of Postgraduate Medical Education and Research, Pondicherry, India.
4. Senior Resident, Department of Pathology, Jawaharlal Institute of Postgraduate Medical Education and Research, Pondicherry, India.
5. Professor, Department of Pathology, Jawaharlal Institute of Postgraduate Medical Education and Research, Pondicherry, India.
Correspondence
Dr. Biswajit Dey,
Senior Resident, Department of Pathology, JIPMER, Pondicherry-605006, India.
E-mail: drbish25@rediffmail.com
Extraskeletal Ewing’s sarcoma is an aggressive malignant small round cell tumour usually occuring in children and adolescents. It needs to be differentiated from other malignant small round cell tumours and immunohistochemistry plays a pivotal role in establishing the diagnosis. Fluorescence in situ hybridization or real time-polymerase chain reaction helps in confirming the diagnosis by demonstration of EWS-FLI1 translocation, which is found in approximately 85% of the cases. We report a case of extraskeletal Ewing’s sarcoma in a10-year-old male, who presented with a right gluteal region mass. Fine needle aspiration and cell block preparation followed by a panel of immunohistochemical markers were performed. Immunohistochemistry for CD99 and FLI1 was positive. EWS-FLI1 translocation was confirmed by fluorescence in situ hybridization.
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